Introduction of food plants developed by genetic engineering is not desirable because
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Solution
Plants developed by genetic engineering are called transgenic plants or genetically modified crops from which genetically modified food is produced. For their production micro-organisms (bacteria, virus) are used.So, by consuming them there is a danger of entry of viruses and toxins causing differ types of allergies and other health hazards to human beings.
Which one of the following represents a palindromic sequence in DNA?
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Solution
A palindromic sequence is a nucleic acid sequence (DNA or RNA) that is the same whether read 5' (five-prime) to 3' (three prime) on one strand or 5' to 3' on the complementary strand with which it forms a double helix.
5. - GAATTC - 3.
3. - CTTAAG - 5.
It is a palindromic sequence of DNA cut by restriction enzyme EcoRI.
The first clinical gene therapy was given for treating
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Solution
Gene therapy is an experimental technique that uses genes to treat or prevent disease. The first clinical gene therapy was given for treating adenosine deaminase deficiency. A four-year old girl became the first gene therapy patient on September 14, 1990 at the NIH Clinical Center. Adenosine deaminase deficiency, also called ADA deficiency or ADA-SCID is an autosomal recessive metabolic disorder that causes immunodeficiency. ADA deficiency is due to a lack of the enzyme adenosine deaminase.
Which of the following forms the basis of DNA fingerprinting?
In genetic engineering, the antibiotics are used
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Solution
Antibiotics are powerful medicines that fight bacterial infections. They either kill bacteria or keep them from reproducing. In genetic engineering, the antibiotics are used as selectable markers.
DIRECTIONS : Each questions contain STATEMENT-1 (Assertion) and STATEMENT-2 (Reason). Each question has 4 choices (a), (b), (c) and (d) out of which ONLY ONE is correct.
Statement 1 : In recombinant DNA technology, human genes are often transferred into bacteria(prokaryotes) or yeast(eukaryote).
Statement 2 : Both bacteria and yeast multiply very fast to form huge population which express the desired gene.
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Solution
Bacteria and yeast are easily grow in culture medium and multiply very fast so it is best for making the many copies of recombinant DNA, and express character of desired gene.
DIRECTIONS : Each questions contain STATEMENT-1 (Assertion) and STATEMENT-2 (Reason). Each question has 4 choices (a), (b), (c) and (d) out of which ONLY ONE is correct.
Statement 1 : Interferons are effective against viruses.
Statement 2 : Proteins which can be synthesized only by genetic engineering are effective against viruses.
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Solution
Interferons are proteins that are effective against most viruses. They are naturally produced by virus infected cells. The proteins interact with adjacent cells and make them resistant to virus attack. Now interferons are also being manufactured through genetic engineering. Interferons control the multiplication of virus particles by inhibiting their protein synthesis.
Molecular probes used for identification of recombinant clone carrying the desired DNA insert can be
(1) denatured double stranded DNA probes
(2) double stranded RNA probes
(3) protein probes
(4) single stranded DNA probes.
Which of the following is/are used in recombinant DNA technology?
(1) agarose gel
(2) ethidium bromide
(3) plasmid vector
(4) restriction endonuclease
Which of the following is/are part(s) of biotechnology?
(1) in vitro fertilisation
(2) synthesis of a gene
(3) correcting a defective gene
(4) developing a DNA vaccine