Which one of the following option is correct for A, B, C and D ?

A-Exonuclease; B-Endonuclease; C-DNA ligase; D-Transformation
A-Exonuclease; B-Exonuclease; C-DNA ligase; D-Transformation
A-Exonuclease; B-Endonuclease; C-Hydrolase; D-Transduction
A-Restriction endonuclease; B-Restriction endonuclease; C-DNA ligase; D-Transformation

A bioreactor refers to

Solution

In industries, fermentation is carried out in special devices (fermentation tank) called bioreacters.

DNA finger printing technique was first developed by

Solution

DNA finger printing technique was first developed by Alec Jeffreys, Wilson and Thien in 1984. This technique is very useful for DNA test for identity and relationships,forensic studies are polymorphism.

There is a restriction endonuclease called EcoRI. What does co. part in it stand for ?

Solution

EcoRI is an endonuclease enzyme isolated from strains of E.coli and a part of restriction modified system. So co part stands for coli.

Restriction endonucleases are enzymes which

Solution

Restriction endonucleases are enzymes that makes cuts at specific positions within the DNA molecule.They acts as molecular scissors. They recognise specific base sequence at palindrome sites in DNA duplex and cut its strands.

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

Solution

Palindromic sequences in DNA molecule are group of bases that forms the same sequence when read in both forward and backward direction. In the given question, only option (c) represent a palindromic sequence.

Polyethylene glycol method is used for

Solution

Direct gene transfer is the transfer of naked. DNA into plant cells but the presence of rigid plant cell wall acts as a barrier to uptake. Therefore protoplasts are the favoured target for direct gene transfer. Polyethylene glycol mediated DNA uptake is a direct gene transfer method that utilizes the interaction between polyethylene glycol, naked DNA, salts and the protoplast membrane to effect transport of the DNA into the cytoplasm.

The linking of antibiotic resistance gene with the plasmid vector became possible with

Solution

The linking of antibiotic resistance gene with the plasmid vector became possible with DNA ligase. DNA ligase is an enzyme that is able to join together two portions of DNA and therefore plays an important role in DNA repair. DNA ligase is also used in recombinant DNA technology as it ensures that the foreign DNA is bound to the plasmid into which it is incorporated.

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