A biologist intends to use a polymerase chain reaction to perform a genetic task. The biologist probably is trying to

discover new genes.
clone a gene.
cut DNA into many small fragments.
isolate DNA from a living cell.

Solution

PCR is away to dramatically increase the numbers of a particular sequence of DNA (or an entire gene). This technique is most useful when cloning DNA since prior knowledge of the DNA sequence must first be obtained.

In genetic engineering, genes can be inserted from one organism into another or back into the original organism using which of the following techniques?

Polymerase chain reaction
Gene gun
DNA hybridization
Gel electrophoresis

Solution

Besides the gene gun, other methods of genetic transfer between cells or organisms include the use of vectors such as viruses and plasmids.

Which of the following is not necessary to execute a polymerase chain reaction successfully?

All four DNA bases
Short DNA base primers
DNA polymerase
DNA library

Solution

Besides the gene gun, other methods of genetic transfer between cells or organisms include the use of vectors such as viruses and plasmids.

Identify the correct match for the given apparatus.

biology-screen-1

Apparatus	Function
Gene gun	Vectorless direct gene transfer
Column chromatograph	Separation of chlorophyll pigments
Stirred tank bioreactor	Carry out fermentation process
Respirometer	Finding out rate of respiration

Recombinant DNA molecules were first of all synthesized by Paul Bery using DNA of

TMV and Salmonella
Virus SB-40 and E.coli
Influenza virus and Diplococcus bacterium
TMV and Agro-bacterium tumefaciens

PCR and Restriction Fragment Length Polymorphism are the methods for :

Study of enzymes
Genetic transformation
DNA sequencing
Genetic Finger printing

A single strand of nucleic acid tagged with a radioactive molecule is called :

Vector
Selectable marker
Plasmid
Probe

Solution

A single strand DNA or RNA tagged with radioactive molecule that is used in of hybridization of DNA or RNA is called probe.

Which one is a true statement regarding DNA polymerase used in PCR

It is used to ligate introduced DNA in recipient cell
It serves as a selectable marker
It is isolated from a virus
It remains active at high temperature

Solution

Taq polymerase are extracted from a thermophilic bacterial and so reman active at high temperature.

For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of :

Silver or Platinum
Platinum or Zinc
Silicon or Platinum
Gold or Tungsten

Solution

For gene transfer into the host cell without using vector micro particles made of tungsten and Gold coated with foregin DNA are bombarded into target cells at a very high velocity.

Biolistics (gene-gun) is suitable for

DNA finger printing.
Disarming pathogen vectors.
Transformation of plant cells.
Constructing recombinant DNA molecules

Solution

Biolistic it is direct gene transferred method for constructing recombinant DNA.

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