A genetic marker is a(n)

place where a restriction enzyme cuts DNA.
chart that traces the family history of a genetic trait.
nucleotide sequence near a particular gene.
radioactive probe used to find a gene

Expression vectors are different from other vectors because they

contain drug resistance markers.
contain telomeres.
contain regulatory regions that permit the cloned DNA to produce a gene product.
contain DNA origins.

Solution

Expression vectors may contain drug resistance markers,telomeres, and must contain DNA origins but none of these features distinguish them from other vectors.Expression vectors are unique because they contain regulatory sequences that allow the cloned gene to be expressed in the host cell.

A genomic library is

where you look to find out howto make recombinant DNA.
a listing of the known nucleotide sequences for a particular species.
all the genes contained in one kind of cell.
a collection of cloned DNA pieces from an organism's genome.

Solution

a collection of cloned DNA pieces from an organism's genome.

Electrophoresis is used to

separate fragments of DNA.
clone genes.
cut DNA into fragments.
match a gene with its function

Which of the following is not necessary to execute a poly-merase chain reaction successfully?

All four DNA bases
Short DNA base primers
DNA polymerase
DNA library

Which of the following statements about restriction enzymes is false?

They work on DNA extracted from all types of organisms.
They are used toglue together short segments of DNA.
They come in many varieties, each with its own DNA target sequence.
They are highly specificfor their DNA target sequences

Solution

Ligases are the enzymes used to glue together DNA fragments.

DNA ligases are enzymes that can beused to(a)chop alarge DNA molecule into small fragments.(b)copy DNA fragments.(c)insert the DNA from one species into the DNA of anotherspecies.(d)separate DNA fragments based on their size

chop alarge DNA molecule into small fragments.
copy DNA fragments.
insert the DNA from one species into the DNA of another species.
separate DNA fragments based on their size

Solution

Ligases are used to connect short DNA fragments to form longer segments, a process essential for inserting DNA segments into vectors.

Imagine a gel through which DNA fragments have moved in response to an applied electrical current. The band on this gel that is farthest from the top (that is, from the place where the DNA fragments were added to the “well”) represents the

shortest fragments of DNA.
longest fragments of DNA.
restriction enzyme used to cut the DNA into fragments.
ligase used to bind the DNA fragments together.

Solution

Migration through, the electrophoresis gel is a function of the size of the DNA fragments, with small fragments moving farthest as they are able to “squeeze” through the gel matrix more easily.

First restriction endonuclease enzyme was discovered in

E. coli
PPLO
Haemophilous influenzae
Bacteriophages

First restriction endonuclease enzyme was discovered in

PPLO
E. coli
Haemophilous influenzae
Bacteriophages

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